Movie 8

Time-lapse movie of cells transiently co-transfected with YFP-IQGAP1, F-Tractin-mTurquoise2, and subsequently stained with WGA-Alexa647, showing a decline of IQGAP1 signal preceding a burst of actin polymerization coinciding with compartment disintegration, and dispersal of several WGA-stained vesicles. 60-second frame rate, 120 min run time, 10 μm scale bar shown.

The coordinating role of IQGAP1 in the regulation of local, endosome-specific actin networks

Edward B. Samson, David S. Tsao, Jan Zimak, R. Tyler McLaughlin, Nicholaus J. Trenton, Emily M. Mace, Jordan S. Orange, Volker Schweikhard, and Michael R. Diehl

Biology Open 2017. 6:785-799; doi: 10.1242/bio.022624