Movie 8

Time-lapse movie of cells transiently co-transfected with YFP-IQGAP1, F-Tractin-mTurquoise2, and subsequently stained with WGA-Alexa647, showing a decline of IQGAP1 signal preceding a burst of actin polymerization coinciding with compartment disintegration, and dispersal of several WGA-stained vesicles. 60-second frame rate, 120 min run time, 10 μm scale bar shown.