CAPNS1 depletion is coupled to an accumulation of LC3-II-positive structures. Control (Movie 1) and shCAPNS1 U2OS cells (Movie 2) were incubated with commercial baculoviruses expressing RFP-GFP-LC3. 24 hours later, a 2 hours time-lapse experiment was performed using a confocal microscope. After the first 15 minutes of images acquisition, 100 nM thapsigargin was added to the cells. Images were acquired every two minutes.