Movie 7

Representative embryo treated with CPA exhibiting extended Ca²⁺ elevation. In contrast with controls, embryos treated with CPA, with or without PMA, featured prolonged elevations of Ca²⁺. Many Ca²⁺ impulses are of normal duration but at the start of the video, a white arrow marks the start of a prolonged Ca²⁺ impulse, with a grey arrow indicating its return to baseline. Scale bar: 50 μm.