Movie 8

Embryo treated with CPA+PMA demonstrates a “hyper-migration” phenotype. Where branching was rescued, either by withdrawing CPA or adding PMA, static imaging revealed some embryos with excessive sprouting and even supernumerary tracheal trunks. This movie shows one such CPA+PMA-treated embryo which reveals a "hyper-migration" phenotype. Arrows mark collections of tracheal cells that migrate away from one dorsal branch to join adjacent branches and even form a disorganized auxiliary trunk and network. Examples of propagating Ca²⁺ impulses are also apparent in this late-stage 16 embryo. Scale bar: 50 μm.