Movie 1

Dynamic changes of F-actin during cell extrusion.
The center of an epithelial cell in the Lifeact-GFP–overexpressing embryo was directly irradiated with a single pulse of the femtosecond laser (15 nJ/pulse) at time = 0 s. Scale bar: 10 μm. F-actin ring was generated at around 120 s, and subsequently tightened.