Movie 2

Dynamic changes of myosin II during cell extrusion.
The center of an epithelial cell in the MRLC-GFP–overexpressing embryo was directly irradiated with a single pulse of the femtosecond laser (15 nJ/pulse) at time = 0 s. Scale bar: 10 μm. Myosin II accumulated at the membranes of cells surrounding the dying cell at around 120 s, and persisted throughout cell extrusion.

Quantitative analysis of mechanical force required for cell extrusion in zebrafish embryonic epithelia

Sohei Yamada, Takanori Iino, Yasumasa Bessho, Yoichiroh Hosokawa, and Takaaki Matsui

Biology Open 2017. 6:1575-1580; doi: 10.1242/bio.027847