Movie 4

Effect of Y27632 for cell extrusion.
Lifeact-GFP–overexpressing embryos were treated with Y27632 for 1 h. The center of an epithelial cell in the Y27632-treated embryo was directly irradiated with a single pulse of the femtosecond laser (15 nJ/pulse) at time = 0 s. Under this condition, actomyosin ring formation occurred, but cell extrusion was delayed. Scale bar: 10 μm.