Movie 5

Effect of Blebbistatin for cell extrusion.
Lifeact-GFP–overexpressing embryos were treated with Blebbistatin for 1 h. The center of an epithelial cell in the Blebbistatin-treated embryo was directly irradiated with a single pulse of the femtosecond laser (15 nJ/pulse) at time = 0 s. Under this condition, actomyosin ring formation occurred, but cell extrusion was delayed. Scale bar: 10 μm.

Quantitative analysis of mechanical force required for cell extrusion in zebrafish embryonic epithelia

Sohei Yamada, Takanori Iino, Yasumasa Bessho, Yoichiroh Hosokawa, and Takaaki Matsui

Biology Open 2017. 6:1575-1580; doi: 10.1242/bio.027847