Movie S2

Representative 360-degree horizontal 3-D construction of confocal photomicrographs showing spatial distribution of PECAM-expressing 3-D system-derived E2C+ Bra-GFP+ cells within mouse embryonic kidney rudiments. Rudiments were cultured ex vivo for 5 days. Immunostaining for E2C (blue) and PECAM-1 (green) was performed to identify mesodermal and endothelial-like cells, respectively (attached as a separate file).

Functional comparison of distinct Brachyury+ states in a renal differentiation assay

Jing Zhou, Antonius Plagge, and Patricia Murray

Biology Open 2018. 7:None-None; doi: 10.1242/bio.031799