Spindly can be observed moving along tracks on the cell cortex. U2OS cells stably expressing GFP-Spindly were imaged using total internal reflection (TIRF) microscopy. The movie on the left shows the original data. To better visualize moving particles, a temporal median filter was used to highlight particles that moved from frame to frame and a trailing function was used to link particles. The processed image is shown on the right. Movie is shown at 20x realtime. Field of view = 40.72 × 40.72 μm.