Movie S2

Rescue of larval crawling by expression of Arl8 in motor neurons.
Movie of larvae expressing Arl8-GFP under the control of the indicated drivers, which, except for Mef2, fully rescue larval movement and the tail flipping phenotype of the Arl8^KO1/Arl8^KO1 mutant. The fly lines used were: w^-; P{W⁺ UAST-Arl8 GFP}/Cyo; PBac{RB}Gie^e00336/ TM6B; w^-; act5C GAL4/Cyo; PBac{RB}Gie^e00336/ TM6B; w^-; n-syb-GAL4/Cyo; PBac{RB}Gie^e00336/ TM6B; w^-; C164- GAL4/Cyo; PBac{RB}Gie^e00336/ TM6B and w^-; Mef2 GAL4/Cyo; PBac{RB}Gie^e00336/ TM6B.