Movie S1

Live cell single molecule video of CHOK1 cell transfected with mEos2 α_vβ₃imaged at 25 ms per frame for 2 min (4800 frames). Red circles indicate detected molecules. Interspersed every 10s (400 frames) is an EGFP fill which allows determination of the cell outline. Field size – 28.4μm x 28.4μm.