Movie 1

Particle sedimentation after agitation in regular and column-augmented wells of 6-well tissue culture plate. Silicone beads (ThermoFisher) of 4 ìm diameter were suspended in 3 ml PBS in a 1:5 volumetric dilution and transferred into regular and column-augmented wells. Tissue culture plate then was placed on horizontal shaker platform and agitated at 250 rpm for 3 seconds. While beads visibly collected to the central region of the regular well as an result of fluid movement, an even flow and sedimentation was observed in column-augmented wells.

A solution to prevent secondary flow in adherent cell cultures

Peter Szaraz, Matthew Librach, Poonam Mander, Banafshe Hoseini, Max Librach, Farwah Iqbal, and Clifford Librach

Biology Open 2019. 8:None-None; doi: 10.1242/bio.045294