Movie 3

Mitochondrial calcium dynamics in control and Drp1 embryos during wound closure. Embryos express the mitochondrial Ca²⁺ indicator mito::GCaMP3. Images are maximum Z-projections of 35 slices (17.5-μm-thick stack) acquired by time-lapse spinning-disk confocal microscopy every 30s and pseudo-colored in a gradient (ranging from lower intensities in blue to higher intensities in yellow). The frame rate is five frames/s. Video stills are shown in Fig. 4.