Movie 2

hemizygous proslong-REDr flies phenocopy the homozygous proslong-REDr adult activity defect
1-3 day old mixed sex flies were transferred to empty vials and allowed to acclimatise for two hours without disturbance. The vial on the left contains wild type flies and the vial on the right contains proslong-REDr/Df(3R)Exel7308 flies. The deficiency (Df(3R)Exel7308) includes the entire pros gene region. After disturbance, by tapping the vial to bring all flies to the bottom, the wild type flies immediately crawl up the sides of the vial. The activity of the hemizygous proslong-REDr flies is impaired, similarly to the proslong-REDr flies in Movie 1. This result suggests that the phenotype of proslong-REDr is not due to a CRISPR off-target effect.