Movie 2.

Fluorescence live imaging of double homozygous Gruul #8 embryos during blastoderm formation in two channels using light sheet fluorescence microscopy. This hybrid subline expresses mEmerald-labeled histone H2B under control of the tubulin alpha 1-like protein promoter and mRuby2-labeled tubulin alpha 1-like protein under control of a shortened version of the elongation factor 1-alpha promoter. Comparison of time series of one embryo from each flavor proceeding through the 10th, 11th, and 12th synchronous division waves. All flavors show similar fluorescence patterns.

Next generation marker-based vector concepts for rapid and unambiguous identification of single and double homozygous transgenic organisms

Frederic Strobl, Julia Ratke, Franziska Krämer, Ana Utta, Sigrun Becker, and Ernst H. K. Stelzer

Biology Open 2023. 12:None-None; doi: 10.1242/bio.060015