Fluorescence live imaging of double homozygous Gruul #8 embryos during blastoderm formation in two channels using light sheet fluorescence microscopy. This hybrid subline expresses mEmerald-labeled histone H2B under control of the tubulin alpha 1-like protein promoter and mRuby2-labeled tubulin alpha 1-like protein under control of a shortened version of the elongation factor 1-alpha promoter. Comparison of time series of one embryo from each flavor proceeding through the 10th, 11th, and 12th synchronous division waves. All flavors show similar fluorescence patterns.