Movie 2.

Purkinje cells in cc2d2a mutant Tg(tagRFP-T:PC:GCaMP5G) fish at 11-12 wpf. MesoSPIM fluorescence 3D maximum intensity projection of tagRFP-T-positive Purkinje cells in the cerebellum of Tg(tagRFP-T:PC:GCaMP5G) 11-12 wpf zygotic cc2d2a mutant fish, generated using Imaris. The morphology of the Purkinje cell layer is comparable between control and mutant fish. The cerebellum is orientated with anterior to the left. Sale bar is 500 μm.