Movie 4.

Four DMZs migrating on a fibronectin coated substrate in the round (ITR). Actin (greyscale) was labeled by injection of mRNA encoding LifeAct-RFP. Two of the four DMZs were labeled with Alexa 488 dextran (magenta-left panel) to identify individual DMZs. Confocal Z-stacks were collected at 2 min intervals (25 frames). The debris in the center of the 4 DMZs is yolk granules released from the cells during microdissection.