Interface between two DMZs in vitro (left) and in silico (right) migrating from 4 DMZs placed ITR. Left: Actin (greyscale) was labeled by injection of mRNA encoding LifeAct-RFP. Two of the four DMZs were labeled with Alexa 488 dextran (magenta-left panel) to identify individual DMZs. Confocal Z-stacks were collected at 3 min intervals (11 frames). Representative frames from a ITR simulation are shown on the right.