Movie 1.

Phospho-mimic α-cat restrains mitotic rounding. [Related to Fig. 3] Confocal images taken on AXR Nikon microscope of MDCK monolayer fixed and immuno-stained with antibodies to α-cat (green). DNA stained with Hoechst (blue). 4D image visualization on Imaris AI Microscopy Image Analysis Software was threshold, gated for voxels, and surface detail set between 0.2-0.5. Overlay 4D image analysis shows apical extension of nucleus during mitosis. Scale bar = 5μm.