Movie 4

The Tc-dpp^RNAi phenotype after embryonic knockdown at approximately 12h AEL bypasses dorsal-ventral patterning defects but impairs morphogenesis. The transgenic nuclear-GFP embryo is shown in lateral aspect with anterior left and dorsal up, from serosal window closure through early germ band retraction stages. Serosal window closure is wild type, but the anterior amnion underneath the head shows defects, most likely due to an incomplete dissociation from the serosa. In addition, the posterior germ band extension is disturbed, characterized by a transient curling of the abdomen, similar to Tc-Doc embryonic RNAi. Image stacks were acquired every 10 min at 30°C and are shown as maximum intensity projections at each time point.