Tg(Kdrl:Ras-mCherry)s916 embryos were injected with plasmid expressing PKI-GFP fusion under control of endothelial fli1ep promoter and vascular development was monitored from 29 till 42hpf. Membrane-bound mCherry fluorescent protein (black in left panel and red in the right) depicts higher mobility and excessive filopodia formation in PKI-GPF-expressing cells (green in the right panel) compared to the wild type GFP-negative cells. Time frame: 25min.
Pavel I. Nedvetsky, Xiaocheng Zhao, Thomas Mathivet, Irene M. Aspalter, Fabio Stanchi, Ross J. Metzger, Keith E. Mostov, and Holger Gerhardt
Development
2016. 143:3582-3590; doi: 10.1242/dev.134767