Movie S1

Time-lapse imaging of preimplantation HprtRED/HprtGFP embryos. Morula stage embryos (E2.5) from oviducts were cultured in potassium simplex optimized medium. Using confocal microscopy, images were taken at 15-min intervals with three channels (bright field, 488 and 561 nm) for up to 5 days. For each embryo, 66 images were taken in the Z-axis at 2-μm intervals for a total depth of ~140 μm.

Live imaging of X chromosome reactivation dynamics in early mouse development can discriminate naïve from primed pluripotent stem cells

Shin Kobayashi, Yusuke Hosoi, Hirosuke Shiura, Kazuo Yamagata, Saori Takahashi, Yoshitaka Fujihara, Takashi Kohda, Masaru Okabe, and Fumitoshi Ishino

Development 2016. 143:2958-2964; doi: 10.1242/dev.136739