Movie 2

Live imaging of early Tc-capicua RNAi embryo
12 hours Time-lapse fluorescence recording of a Tribolium embryo expressing nuclearlocalized GFP under a ubiquitous promoter (transgenic line EFA-nGFP). Stacks were recorded every 12 minutes at 10x magnification and 20°C. Upon Tc-cic depletion, the serosa anlagen expands at the expense of the anterior head anlagen. Eventually extraembryonic membranes cover the entire egg. Embryonic tissue is restricted to the posterior pole of the egg and becomes internalized completely. Anterior to the left.