Movie 3

Live imaging of early Tc-maelstrom RNAi embryo
12 hours Time-lapse fluorescence recording of a Tribolium embryo expressing nuclearlocalized GFP under a ubiquitous promoter (transgenic line EFA-nGFP). Stacks were recorded every 12 minutes at 10x magnification and 20°C. Upon Tc-mael RNAi, head anlagen are expanded and less condensed as compared to wildtype. Serosa window formation remains incomplete to some degree and RNAi posterior elongation fails. Eventually, the serosa ruptures premature. Anterior to the left.

Transcriptome sequencing reveals maelstrom as a novel target gene of the terminal system in the red flour beetle Tribolium castaneum

Fabian Pridöhl, Matthias Weißkopf, Nikolaus Koniszewski, Andreas Sulzmaier, Steffen Uebe, Arif B. Ekici, and Michael Schoppmeier

Development 2017. 144:1339-1349; doi: 10.1242/dev.136853