Movie 2

Apical constriction is not affected in CMO-containing neural plate cells. Two-cell-stage embryos were bilaterally injected with membrane-GFP (in green) and unilaterally with 10 pmol CMO + AlexaFluor 594-dextran conjugate (in red). Confocal images of the apical neural plate in live embryos at stage 15-15.5 were taken every 5 min. Shown are z-projections of the sequence of time frames during 1 h recording. Sample realignment was done after 30-min recording to recenter the field of view on the neural plate midline. Note decrease in cell surface and folding of the neural plate in the wild-type (top, green) and CMO-containing neural plate (bottom, yellow).