Supplemental Movie 2

Microtubule gliding assay with mutant (DYNC1H1^D/V) immobilised recombinant human dynein.
Representative time-lapse movie of gliding of 543-labelled microtubules by surface-immobilised GFP-dynein with GFP antibodies (GFP channel not shown). This assay was performed in 30 mM HEPES, 5 mM MgSO4, 1 mM DTT, 1 mM EGTA, 40 μM taxol, 1 mg/ml á-casein, 2.5mM ATP, pH 7.0).