FRAP analysis of GFP-actin in control bristles at 40 h APF.
FRAP analysis of GFP-actin-expressing control bristles at 40 h APF. GFP-actin fluorescence did not recover within the time course of this movie, suggesting that actin bundles are relatively stable. Images were acquired at 3 s intervals for 5 min, with 6 z-sections at 1.5 µm intervals.
Tetsuhisa Otani, Yosuke Ogura, Kazuyo Misaki, Takuya Maeda, Akiyo Kimpara, Shigenobu Yonemura, and Shigeo Hayashi
Development
2016. 143:3806-3816; doi: 10.1242/dev.138495