Movie S4

FRAP analysis of Fascin[S52E]-GFP in control bristles at 40 h APF.
FRAP analysis of Fascin[S52E]-GFP-expressing control bristles at 40 h APF. Fascin[S52E]-GFP fluorescence recovered rapidly after photobleaching, suggesting that Fascin dynamically exchanges in the actin bundles. Images were acquired at 3 s intervals for 5 min, with 4 z-sections at 1.5 µm intervals.

IKKε inhibits PKC to promote Fascin-dependent actin bundling

Tetsuhisa Otani, Yosuke Ogura, Kazuyo Misaki, Takuya Maeda, Akiyo Kimpara, Shigenobu Yonemura, and Shigeo Hayashi

Development 2016. 143:3806-3816; doi: 10.1242/dev.138495