Movie 2

Ectopic endoderm is not induced when TAEL injected embryos are kept in the dark. Z-stack through a Tg(cryaa:Venus; 5xC120:sox32); Tg(sox17:GFP) embryo at approximately 70% epiboly. The embryo was injected with H2B-mCherry mRNA to label all nuclei (red) and TAEL mRNA, then kept in the dark until image acquisition. Images were acquired every 0.9 μm on a Nikon Ti-E microscope equipped with a Yokogawa CSU-22 spinning disk confocal unit. The z-stack starts at the embryo surface and goes through towards the yolk. Tg(sox17:GFP) expression (green) appears in only at the innermost layer closest to at the yolk, consistent with endogenous endoderm localization.

TAEL: a zebrafish-optimized optogenetic gene expression system with fine spatial and temporal control

Anna Reade, Laura B. Motta-Mena, Kevin H. Gardner, Didier Y. Stainier, Orion D. Weiner, and Stephanie Woo

Development 2017. 144:345-355; doi: 10.1242/dev.139238