Motility of wild-type spermatozoa. Spermatozoa were isolated from cauda epididymis of adult testis (19 weeks old) in HTF medium containing 0.5% methyl cellulose to increase viscosity and slow down sperm cells. Flagella beat vigorously and efficiently propel wild-type sperm cells. A cytoplasmic droplet, involved in the final removal of excess cytoplasm, is visible in the distal midpiece of some spermatozoa.
Marina Weidemann, Karin Schuster-Gossler, Michael Stauber, Christoph Wrede, Jan Hegermann, Tim Ott, Karsten Boldt, Tina Beyer, Katrin Serth, Elisabeth Kremmer, Martin Blum, Marius Ueffing, and Achim Gossler
Development
2016. 143:4736-4748; doi: 10.1242/dev.139626