Movie 1

Anastomosis in a wild-type embryo
Deconvolved spinning disc confocal movie of a Tg(fli1ep:gal4ff)ubs3, Tg(UAS:EGFP-UCHD)ubs18, Tg(kdrl:mCherry-CAAX)s916 embryo at around 32hpf, anterior to the left. Confocal stacks were acquired every minute. Single channels are shown in inversed contrast (green is EGFP-UCHD and red is mCherry-CAAX on left and right, respectively) and the merged channels are shown in the middle. Two tip cells expand filopodia towards each other. Few transient filopodial interactions are observed before a single filopodial contact is maintained and reinforced by the recruitment of actin cytoskeletal components (around the 84th minute of the movie). The cell-cell contact matures and the actin cytoskeleton reorganizes to form junctional ring between the two tip cells (around 129th minute of the movie).

Distinct and redundant functions of Esama and VE-cadherin during vascular morphogenesis

Loïc Sauteur, Markus Affolter, and Heinz-Georg Belting

Development 2017. 144:1554-1565; doi: 10.1242/dev.140038