Angiogenesis in a wild-type embryo
Confocal movie of a Tg(fli1a:EGFP)y1 embryo starting around 26hpf (= 0min) shown in inversed contrast, anterior to the left. Confocal stacks were acquired every 10 minutes. The angiogenic sprouts expand dorsally and the first anastomotic contacts are observed around 31hpf (5h of the movie). As angiogenesis progresses lumen is pushed through the SAs into the DLAV (12h of the movie). At the end of the movie (~44hpf), all the SAs and the DLAV are lumenized.