Movie 5

Angiogenesis in an esama^ubs19 mutant embryo
Confocal movie of a Tg(fli1a:EGFP)^y1 embryo starting around 26hpf (= 0min) shown in inversed contrast, anterior to the left. Confocal stacks were acquired every 10 minutes. Angiogenesis in esama^ubs19 mutant embryo progresses indistinguishable from wild-type embryos. The angiogenic sprouts expand dorsally and the first anastomotic contacts are observed around 30hpf (4h of the movie). As angiogenesis progresses lumen is pushed through the SAs into the DLAV (12h of the movie). At the end of the movie (~44hpf), all the SAs and the DLAV are lumenized.