Movie 6

Angiogenesis in a ve-cadubs8 mutant embryo
Confocal movie of a Tg(fli1a:EGFP)^y1 embryo starting around 29hpf (26hpf = 0min) shown in inversed contrast, anterior to the left. Confocal stacks were acquired every 8 minutes. Tip cells of angiogenic sprouts start to anastomose around 30hpf (around 4h of the movie), which appears less efficient compared to wild-type embryos. In 34% of the SAs we observe detachments of tip cells from stalk cells, this examples however does not show this phenotype. Nevertheless, a basic vascular network, i.e. SAs and the DLAV, is formed. No lumen is pushed through the vessels due to the absence of blood pressure. However, sometimes lumen is observed in SAs up to the level of the horizontal myoseptum. The DA is mostly collapsed but may also show inflated portions.