Movie 9

Anastomosis in an esama^ubs19; ve-cad^+/ubs8 mutant embryo
Deconvolved spinning disc confocal movie of a Tg(fli1ep:gal4ff)^ubs3, Tg(UAS:EGFP-UCHD)^ubs18, Tg(kdrl:mCherry-CAAX)^s916 esama^ubs19; ve-cad^+/ubs8 mutant embryo at around 32hpf, anterior to the left. Confocal stacks were acquired every minute. Single channels are shown in inversed contrast (green is EGFP-UCHD and red is mCherry-CAAX on left and right, respectively) and the merged channels are shown in the middle. Similar to ve-cad mutant embryos, many transient filopodial interactions are observed. Only as the tip cell bodies are getting closer to each other and start to overlap (around the 66^th minute) the filopodia/cell bodies do not retract and form an anastomotic contact.