Movie 11

Spontaneous muscle contractions Single plane spinning disc confocal movies of myotubes labelled with Mef2-Gal4, UAS-Lifeact-Ruby and UAS-GCaMP6 at 46 h (upper movie), 50 h (middle) and 52 h APF (lower movie). Spontaneous Ca²⁺ influx is found at all stages, and induces a small contraction at 46 h APF and strong contractions at 50 h and 52 h APF. Time is indicated in seconds. Movies plays with 5 frames per second.