Myotubes at 46 h but not 40 h APF are contractile upon laser lesion Single plane spinning disc confocal movies of myotubes labelled by Mef2-GAL4, UAS-GFP-Gma at 40 h (upper movie) and 46 h APF (lower movie). The muscles in the center of the movies were severed by a UV nano-lesion (see Figure 6 A, B). Note the induced contraction after the cut at 46 h but not at 40 h APF. Time is indicated in seconds and starts at the cut. Movies plays with 5 frames per second.