Laser severing induces Ca2+ release Single plane spinning disc confocal movies of myotubes labelled with Mef2-GAL4, UAS-GCaMP6 at 40 h (upper movie) and 46 h APF (lower movie). Both muscles were severed by a UV nano-lesion (see Figure 6 E, F). Note the induced Ca2+ release at both time points, with induced contraction only at 46 h APF. Time is indicated in seconds and starts at the cut. Movies plays with 10 frames per second.