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Movie 1

Venus::CYK-1 (yellow) is cytoplasmic and is also found in dynamic juxta-apical punctae in wild-type. Arrows mark the first appearance of punctae that were visible for at least two frames (~90 seconds). In this ~10-minute movie we counted at least 12 such punctae. F-actin, labelled with LifeAct::TagRFP (magenta), is found at the lumen-lining apical membrane, and can be seen accumulating in some of the juxta-apical Venus::CYK-1 labelled punctae. This movie is a maximum projection of seventeen 1μm z-steps taken using the settings for Venus::CYK-1 described in the “Spinning Disk Microscopy Settings“ section of the Supplementary Materials and Methods.