Movie 2

3D projection of the same wild-type EC seen in Fig. 2F, showing that that when not present in juxta-apical punctae, Venus::CYK-1 appears cytoplasmic and reaches the basolateral side (entire cytoplasm is marked by CFP). Eight 1.0μm thick sections were used to generate this projection, and images were acquired as described for Venus::CYK-1 in the “Spinning Disk Microscopy Settings” section of the Supplementary Materials and Methods.