Intracellular Ca2+ dynamics in the neural plate. An embryo expressing R-GECO1.0 was imaged during the closure of the neural tube. Imaging shows a dorsal view of the presumptive anterior spinal cord region; anterior is to the top. Elapsed time (minutes: seconds) is shown at the top left. Frames were captured every 20 seconds using spinning-disk confocal microscopy. Movie frame rate: 20 frames/sec.