Effect of E-NTPDase1 on intracellular Ca2+ dynamics. Embryos expressing R-GECO1.0 without (left) and with E-NTPDase1 (right) were imaged during neural tube closure. Images show a dorsal view of the presumptive anterior spinal cord region; anterior is to the top. The elapsed time (minutes: seconds) is shown at the top left. Frames were captured every 40 seconds using spinning-disk confocal microscopy. Movie frame rate: 15 frames/sec.