Dynamics of F-actin and intracellular Ca2+ in the neural plate. An embryo expressing Lifeact-EGFP (left) and R-GECO1.0 (right) was imaged during neural tube closure. Images show a dorsal view of the presumptive anterior spinal cord region; anterior is to the top. Elapsed time (minutes: seconds) is shown at the top left. Frames were captured every 20 seconds using spinning-disk confocal microscopy. Movie frame rate: 15 frames/sec.