Movie 4

Dynamics of F-actin and intracellular Ca2+ in the neural plate. An embryo expressing Lifeact-EGFP (left) and R-GECO1.0 (right) was imaged during neural tube closure. Images show a dorsal view of the presumptive anterior spinal cord region; anterior is to the top. Elapsed time (minutes: seconds) is shown at the top left. Frames were captured every 20 seconds using spinning-disk confocal microscopy. Movie frame rate: 15 frames/sec.

Distinct intracellular Ca2+ dynamics regulate apical constriction and differentially contribute to neural tube closure

Makoto Suzuki, Masanao Sato, Hiroshi Koyama, Yusuke Hara, Kentaro Hayashi, Naoko Yasue, Hiromi Imamura, Toshihiko Fujimori, Takeharu Nagai, Robert E. Campbell, and Naoto Ueno

Development 2017. 144:1307-1316; doi: 10.1242/dev.141952