Photolysis of NPE-caged IP3 in the neural plate. An embryo expressing EGFP-CAAX (left) and R-GECO1.0 (right) was imaged during neural tube closure. Imaging shows a dorsal view of the presumptive anterior spinal cord region; anterior is to the top. Elapsed time (seconds) is shown at the top left. A UV light was shone onto a central circular region for 120–125 seconds. Frames were captured every 5 seconds using spinning-disk confocal microscopy. Movie frame rate: 15 frames/sec.
Makoto Suzuki, Masanao Sato, Hiroshi Koyama, Yusuke Hara, Kentaro Hayashi, Naoko Yasue, Hiromi Imamura, Toshihiko Fujimori, Takeharu Nagai, Robert E. Campbell, and Naoto Ueno
Development
2017. 144:1307-1316; doi: 10.1242/dev.141952