Photolysis of NPE-caged IP3 in the neural plate. An embryo expressing EGFP-CAAX (left) and R-GECO1.0 (right) was imaged during neural tube closure. Imaging shows a dorsal view of the presumptive anterior spinal cord region; anterior is to the top. Elapsed time (seconds) is shown at the top left. A UV light was shone onto a central circular region for 120–125 seconds. Frames were captured every 5 seconds using spinning-disk confocal microscopy. Movie frame rate: 15 frames/sec.