Movie 5

Photolysis of NPE-caged IP3 in the neural plate. An embryo expressing EGFP-CAAX (left) and R-GECO1.0 (right) was imaged during neural tube closure. Imaging shows a dorsal view of the presumptive anterior spinal cord region; anterior is to the top. Elapsed time (seconds) is shown at the top left. A UV light was shone onto a central circular region for 120–125 seconds. Frames were captured every 5 seconds using spinning-disk confocal microscopy. Movie frame rate: 15 frames/sec.

Distinct intracellular Ca2+ dynamics regulate apical constriction and differentially contribute to neural tube closure

Makoto Suzuki, Masanao Sato, Hiroshi Koyama, Yusuke Hara, Kentaro Hayashi, Naoko Yasue, Hiromi Imamura, Toshihiko Fujimori, Takeharu Nagai, Robert E. Campbell, and Naoto Ueno

Development 2017. 144:1307-1316; doi: 10.1242/dev.141952