Movie 9

Effect of photolysis of DMNPE-caged ATP on the neural plate of an embryo injected with N-cadherin-Mo. An N-cadherin-Mo-injected embryo expressing EGFP-CAAX (left) and R-GECO1.0 (right) in DMNPE-caged ATP-containing ringer solution was imaged during the neural tube closure. Dorsal view of the presumptive anterior spinal cord region; anterior is to the top. Number in the left top indicates time (minutes: seconds). UV light was shone onto a central circular region for 10–30 seconds. Frames were captured every 1 second using spinning-disk confocal microscopy and the movie plays at 15 frames/sec.