Movie 9

Effect of photolysis of DMNPE-caged ATP on the neural plate of an embryo injected with N-cadherin-Mo. An N-cadherin-Mo-injected embryo expressing EGFP-CAAX (left) and R-GECO1.0 (right) in DMNPE-caged ATP-containing ringer solution was imaged during the neural tube closure. Dorsal view of the presumptive anterior spinal cord region; anterior is to the top. Number in the left top indicates time (minutes: seconds). UV light was shone onto a central circular region for 10–30 seconds. Frames were captured every 1 second using spinning-disk confocal microscopy and the movie plays at 15 frames/sec.

Distinct intracellular Ca2+ dynamics regulate apical constriction and differentially contribute to neural tube closure

Makoto Suzuki, Masanao Sato, Hiroshi Koyama, Yusuke Hara, Kentaro Hayashi, Naoko Yasue, Hiromi Imamura, Toshihiko Fujimori, Takeharu Nagai, Robert E. Campbell, and Naoto Ueno

Development 2017. 144:1307-1316; doi: 10.1242/dev.141952