Comparison of silicon chamber and FiZD systems with an embryonic kidney organoid. Kidney organoids prepared from E13.5 frozen embryonic kidneys were placed in both culture systems and grown in time-lapse experiment for 3 days. Images were taken every 15 minutes. A stack of 20 z-layers was captured using a 10x/0.45 Zeiss Plan-Apochromat objective, voxel size 0.69x0.69x4.13 µm.
Ulla Saarela, Saad Ullah Akram, Audrey Desgrange, Aleksandra Rak-Raszewska, Jingdong Shan, Silvia Cereghini, Veli-Pekka Ronkainen, Janne Heikkilä, Ilya Skovorodkin, and Seppo J. Vainio
Development
2017. 144:1113-1117; doi: 10.1242/dev.142950