Movie 10

Comparison of silicon chamber and FiZD systems with an embryonic kidney organoid. Kidney organoids prepared from E13.5 frozen embryonic kidneys were placed in both culture systems and grown in time-lapse experiment for 3 days. Images were taken every 15 minutes. A stack of 20 z-layers was captured using a 10x/0.45 Zeiss Plan-Apochromat objective, voxel size 0.69x0.69x4.13 µm.

Novel fixed z-direction (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging

Ulla Saarela, Saad Ullah Akram, Audrey Desgrange, Aleksandra Rak-Raszewska, Jingdong Shan, Silvia Cereghini, Veli-Pekka Ronkainen, Janne Heikkilä, Ilya Skovorodkin, and Seppo J. Vainio

Development 2017. 144:1113-1117; doi: 10.1242/dev.142950