Movie 11

mTmG;Wnt4Cre embryonic kidneys cultured on top of Trowell insert. E12.5 kidneys cultured for 3 days on top of the Trowell insert. A stack of 20 z-layers was captured using a 10x/0.45 Zeiss Plan-Apochromat objective, images acquired every 15min. Voxel size 0.69x0.69x4.13 µm.

Novel fixed z-direction (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging

Ulla Saarela, Saad Ullah Akram, Audrey Desgrange, Aleksandra Rak-Raszewska, Jingdong Shan, Silvia Cereghini, Veli-Pekka Ronkainen, Janne Heikkilä, Ilya Skovorodkin, and Seppo J. Vainio

Development 2017. 144:1113-1117; doi: 10.1242/dev.142950

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