Movie 11
mTmG;Wnt4Cre embryonic kidneys cultured on top of Trowell insert. E12.5 kidneys cultured for 3 days on top of the Trowell insert. A stack of 20 z-layers was captured using a 10x/0.45 Zeiss Plan-Apochromat objective, images acquired every 15min. Voxel size 0.69x0.69x4.13 µm.
Novel fixed z-direction (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging
Development 2017. 144:1113-1117; doi: 10.1242/dev.142950