Movie 2

Branching of the UB in the FiZD culture. The embryonic kidneys from E11.5 mTmG; Hoxb7Cre embryos were dissected and cultured in the FiZD. Organogenesis was monitored for 5 days. The generated movie illustrates the Hoxb7Cre induced GFP expression in the cells of the ureteric bud. A stack of 20 z-layers was captured using a 20x/0.8 M27 Zeiss Plan-Apochromat objective, images were acquired every 5 minutes, Voxel size 0.69x0.69x1.82 µm.

Novel fixed z-direction (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging

Ulla Saarela, Saad Ullah Akram, Audrey Desgrange, Aleksandra Rak-Raszewska, Jingdong Shan, Silvia Cereghini, Veli-Pekka Ronkainen, Janne Heikkilä, Ilya Skovorodkin, and Seppo J. Vainio

Development 2017. 144:1113-1117; doi: 10.1242/dev.142950