Movie 4

Computer-assisted cell segmentation and tracking for the FiZD derived image data. The quality of the FiZD captured images is good enough for computer assisted analysis of the renal cell behaviour such cell movement during morphogenesis. The movie shows the movement of kidney cells within the ureteric bud branch tip. Green line marks the separation between kidney stem and branch tip. Red line splits the ureteric bud branch tip in the middle to enable the analysis of movement of cells in both halves of the branch tip. Individual cell boundaries and their tracks are also shown.

Novel fixed z-direction (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging

Ulla Saarela, Saad Ullah Akram, Audrey Desgrange, Aleksandra Rak-Raszewska, Jingdong Shan, Silvia Cereghini, Veli-Pekka Ronkainen, Janne Heikkilä, Ilya Skovorodkin, and Seppo J. Vainio

Development 2017. 144:1113-1117; doi: 10.1242/dev.142950